Gastruloids.png

The Liverpool Development & Stem Cell Lab

Taking quantitative approaches to understand cell-decision making during early development

We use mouse embryonic stem cells (mESCs) to study cell fate decisions in both 2D monolayer, and 3D Gastruloid culture. Gastruloids are aggregates of mESCs generated in non-adherent culture, which are able to effectively recapitulate many of the processes of early mammalian development such as symmetry-breaking, polarisation, gastrulation-like movements and the development of the three embryonic axes. 

Our Research

NFkB.png

Cell signalling dynamics and cell fate

Studying how signals alter the dynamics of proteins in individual embryonic stem cells to modulate pluripotency and the decision to differentiate

Gastruloids.png

Gastruloids & axial pattering

Using gastruloids as a model system to understand how the cells of the embryo integrate chemical and physical inputs to specify cell fate, axial patterning, and tissue morphogenesis

A0_NC3Rs_brandmark_ultramarine_cmyk_edit

3Rs approaches to drug screening and disease modelling

Replacing animal models by developing 3D in vitro approaches to assess drug safety during pregnancy, and model pathogen transfer across the foetal/maternal interface

Resources

It's critical that science is reproducible, not just in our lab, but elsewhere. To help with this, we've compiled a list of the standard protocols, reagents, validated antibodies, and tools that we use

Figures 1_edited.jpg

Gastruloid Protocols

Standard protocols for mESC culture, gastruloid protocols, and reagents

antibody_edited.jpg

Validated Antibodies

To save you the trouble of testing antibodies that won't work, we've compiled a list of the ones we regularly use that work

computer.webp

Code

Useful code for gastruloid analysis, single cell tracking, graphing

Replacement
Refinement
Reduction

Our work is firmly embedded in the 3Rs (Reduction Refinement Replacement of animals in research), utilising and developing novel in vitro model systems and protocols that allow us, and the field, to study early developmental patterning processes without using animal models.

Our NC3Rs funded projects
  • Using Gastruloids to study Left-Right Asymmetry (NC/P001467/1)

  • Assessing drug toxicity on the mammalian embryo with Gastruloids (NC/T002131/1)

 

Our collaborative NC3Rs projects
  • A 3Rs approach to investigate the feto-maternal interface (NC/V001566/1); led by Aras Kadioglu and Marie Yang, Liverpool

  • Exploring the role of matrix encapsulation on early developmental decisions using non-animal sourced hydrogels (NC/W001918/1); led by Cathy Merry, Nottingham

A0_NC3Rs_brandmark_ultramarine_cmyk.jpg
1588-royal-society.jpg

Our Funders

ukri-bbsrc-square-logo_edited.png

CONTACT US

William Henry Duncan Building, 6 West Derby Street

01517949041

Thanks for submitting!